With this system, cells are maintained in an undifferentiated state with virtually no background differentiation, eliminating the need for cell selection. The highly reproducible nature of the system, coupled with its ability to ensure an efficient and predictable growth rate, makes the DEF-CS culture system ideal for the expansion and scale-up of a homogeneous population of iPS cells. Following reprogramming, successful expansion of your pluripotent stem cells is crucial. The DEF-CS culture system, a research-grade system, provides media, a coating reagent, and growth factors for the easy culture of iPS cells as a 2D monolayer.
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The Cellartis DEF-CS 500 Culture System is an easy-to-use, complete culture system for defined and feeder-free ES and iPS cell expansion in a non-colony, 2D-monolayer format.
The Cellartis DEF-CS 500 Culture System is an easy-to-use, complete culture system for defined and feeder-free ES and iPS cell expansion in a non-colony, 2D-monolayer format.
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
Cellartis DEF-CS 500 Basal Medium with Additives is a robust media for efficient expansion of human induced pluripotent stem (iPS) cells in a feeder-free and defined environment. This kit includes Cellartis DEF-CS 500 Basal Medium and Cellartis DEF-CS 500 Additives.
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
Cellartis DEF-CS 500 Culture System is a defined culture system for efficient expansion of undifferentiated human pluripotent stem cells. This kit includes basal medium, coating substrate, and additives.
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
Cellartis DEF-CS 500 COAT-1 is a part of the Cellartis DEF-CS 500 Culture System, a defined culture system for efficient expansion of undifferentiated human pluripotent stem cells. In addition to the coating substrate, the complete System includes basal medium and additives.
Complete kit includes 500 ml of basal medium, additives, and coating compound
Robust system provides high reproducibility, stable growth, and ensures maintenance of a normal karyotype
Cells maintain pluripotency and long-term potential for self-renewal and differentiation
Single-cell culture of human iPS cells
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Transfection and reprogramming
High-throughput screening
Tissue engineering (seeding cells on a scaffold)
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Hanson, C. et al. Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro. Acta ophthalmologica 91, 127&#;130 ().
Kia, R. et al. MicroRNA-122: a novel hepatocyte-enriched in vitro marker of drug-induced cellular toxicity. Toxicol. Sci. 144, 173&#;85 ().
Norrman, K. et al. Distinct gene expression signatures in human embryonic stem cells differentiated towards definitive endoderm at single-cell level. Methods 59, 59&#;70 ().
Sivertsson, L. et al. Hepatic differentiation and maturation of human embryonic stem cells cultured in a perfused three-dimensional bioreactor. Stem Cells Dev. 22, 581&#;594 ().
Ulvestad, M. et al. Drug metabolizing enzyme and transporter protein profiles of hepatocytes derived from human embryonic and induced pluripotent stem cells. Biochem. Pharmacol. 86, 691&#;702 ().
Valton, J. et al. Efficient strategies for TALEN-mediated genome editing in mammalian cell lines. Methods 69, 151&#;170 ().
Zandén, C. et al. Stem cell responses to plasma surface modified electrospun polyurethane scaffolds. NANOMED-NBM 10, 949&#;958 ().
Please see the product&#;s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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