Cells Suitable for Cultivation in Roller Bottles

Cell culture is a fundamental technique in biological and medical research, and roller bottles have emerged as a unique and increasingly important tool in this field.

Cell culture is a fundamental technique in biological and medical research, and roller bottles have emerged as a unique and increasingly important tool in this field. Roller bottles offer several advantages for cell culture, particularly for large-scale production and specific research needs.

Advantages of Roller Bottles:

Ample Growth Space: Roller bottles provide a large surface area for cells to grow, which can significantly enhance cell growth rates and yield. This is especially beneficial for experiments and research requiring large quantities of cells, such as drug screening and toxicology studies.

Prevention of Precipitation: The gentle rotation of roller bottles prevents the precipitation of certain components in the culture medium, ensuring a stable culture environment.

Real-Time Monitoring: Researchers can continuously monitor cell growth and adjust culture conditions as needed. This capability enhances the accuracy and reliability of experiments by allowing precise control over the cell growth process.

Types of Cells Suitable for Roller Bottles:

Adherent Cells: Roller bottles are particularly well-suited for the cultivation of various adherent cell types. These include:

Vero Cells: Commonly used in vaccine production and virus research.
HEK 293 Cells: Widely used in gene expression studies and biotechnology.
CAR-T Cells: Important in immunotherapy and cancer research.
MRC5 Cells: Utilized in vaccine production and virology research.
CEF Cells (Chicken Embryo Fibroblast Cells): Used in virology and vaccine production.
Suspension Cells: Roller bottles are also suitable for static culture of suspension cells such as:

CHO Cells (Chinese Hamster Ovary Cells): Frequently used in biopharmaceutical production.
Insect Cells: Commonly used in recombinant protein production.
BHK21 Cells (Baby Hamster Kidney Cells): Utilized in vaccine production and viral research.
MDCK Cells (Madin-Darby Canine Kidney Cells): Often used in influenza vaccine production.
Conclusion:

Roller bottles are versatile tools in cell culture, suitable for both adherent and suspension cells. Their ability to provide a large growth surface, prevent medium component precipitation, and allow real-time monitoring makes them invaluable in various research and production contexts. As technology advances and application needs diversify, roller bottles will continue to play a crucial role in advancing biological and medical research.